CellLight® Fluorescent Protein Labeling

CellLight® reagents provide the easiest way to label specific structures in live cells with fluorescent proteins. Simply add the reagent to your cells, incubate overnight, and you’re ready to image in the morning. These ready-to-use constructs express fluorescent fusion proteins targeted to specific intracellular structures. The fluorescent protein is introduced using a simple transfection step that doesn’t require molecular biology techniques to carry out—it works like a cell stain.

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Guidelines for using CellLight® reagents for cell structure labeling

When to use CellLight® reagents

Use CellLight® reagents when you want to label specific organelles or intracellular structures in live cells with minimal toxicity or chemical disruption. Follow the behavior of individual cells, multiplex with other CellLight® reagents or organic dyes, and analyze in live cells or after fixation. CellLight® reagents will label mitochondria irrespective of membrane potential and lysosomes irrespective of pH for counterstaining or colocalization studies.

When NOT to use CellLight® reagents

Because CellLight® reagents rarely result in 100% transfection rates they are not appropriate for cell population studies or automated imaging or counting. Transfection rates and conditions may differ by cell type, and an extensive reference list on their uses is provided.

Advantages of labeling with CellLight® reagents

  • Choice of specific intracellular targets
  • Simple add-incubate-and-image protocol for each cell type
  • Multiplex with CellLight® reagents, antibodies, or organic dyes

See the CellLight® Reagents Selection Guide to identify targets and colors for convenient multiplexing and colocalization studies.

CellLight® Reagents Selection Guide
RFP
CFP
Trial size reagents

Cellular Target Preview References GFP
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Actin

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Cytoplasm

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Endoplasmic Reticulum

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Early Endosomes

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Late Endosomes

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Golgi

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Histones (Histone 2B)

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Lysosomes

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MAP4

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Mitochondria

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Nucleus
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Peroxisomes

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Plasma Membrane
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Synaptophysin

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Talin

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Tubulin

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BacMam 2.0 Transduction Control

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BacMam delivery technology
BacMam technology provides you with an efficient transduction system so that you can create relevant cell models to run your assays. Use BacMam technology to label organelles with targeted fluorescent proteins, express drug targets such as ion channels or receptors, or monitor cellular processes with fluorescent protein indicators. You can also create your own custom constructs using BacMam DIY.

Resources

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